Identification of cell barcodes from long-read single-cell RNA-seq with BLAZE

Identification of cell barcodes from long-read single-cell RNA-seq with BLAZE

Blog Article

Abstract Long-read single-cell RNA sequencing (scRNA-seq) enables the quantification of RNA isoforms in individual cells.However, long-read scRNA-seq using the Oxford Nanopore platform has largely relied upon matched short-read data to identify cell barcodes.We introduce BLAZE, which accurately and efficiently identifies 10x cell barcodes using only nanopore alphaville clothing long-read scRNA-seq data.BLAZE outperforms the existing tools and provides an accurate representation of the cells present in long-read scRNA-seq when compared to matched short reads.

BLAZE simplifies long-read scRNA-seq while improving the results, is compatible with downstream tools accepting a cell barcode file, and the gel bottle audrey is available at https://github.com/shimlab/BLAZE.